mRNA Designed to Reduce Immunogenicity
Do you need highly stable, low immunogenicity mRNA for your research and downstream applications? Does your workflow require modified nucleotides, such as N1-methyl-pseudouridine (N1meΨ) or pseudouridine (Ψ)?
CELLSCRIPT™ technologies streamline and optimize your mRNA transcription and downstream translation work. Our innovative dual workflow approach combines:
- INCOGNITO™ Kits for high-yield modified nucleotide mRNA synthesis
- Min-Immune™ Gold Kit for removal of unwanted double-stranded RNA
Watch this presentation to learn about a workflow for producing low-immunogenicity, high-stability mRNA.
Choose Your Technologies for Immunogenicity Reduction
- INCOGNITO™ RNA Transcription Kits & mRNA Production Systems
- Min-Immune™ Gold dsRNA Removal Kit
INCOGNITO™ RNA Transcription Kits and mRNA Production Systems are available with either pseudouridine (Ψ), N1-methyl-pseudouridine (N1meΨ), and/or 5-methyl-cytidine + pseudouridine (5mC/Ψ) nucleotides for incorporation during RNA synthesis. The INCOGNITO™ mScript™ Complete Kits incorporate Min-Immune™ Gold as a workflow component of the kit, or Min-Immune™ Gold can be purchased separately.
Use the interactive selection tools below to choose the kit that’s right for your needs:
Choose from 9 INCOGNITO™ Kits to synthesize RNA or mRNA using modified nucleotides (Ψ, N1meΨ, or 5mC/Ψ) for reduced immunogenicity.
Select Min-Immune™ Gold dsRNA Removal Kit to perform post-transcriptional dsRNA removal. Choose from one of three T7 mScript™ Complete mRNA Production Systems that provide all reagents for transcription, 5’ capping, 3’ poly(A) tailing and Min-Immune™ Gold dsRNA removal from samples in an all-in-one kit.
READ THE APPLICATION NOTE
High efficiency mRNA synthesis and ultra-low immunogenicity achieved with the T7 mScript™ Complete mRNA Production Systems
Optimizing mRNA synthesis, modification, and purification enhances translational efficiency and minimizes innate immune activation. Download and read this Application Note which examines the workflow, efficiency and high suitability of T7 mScript™ Complete mRNA Production Systems for synthesis of high-quality mRNA that is essentially devoid of dsRNA and amenable for use in downstream applications such as mRNA vaccine development and cell and gene therapy research where immunogenicity is a concern.
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INFOGRAPHIC
Create the Perfect mRNA for Translation in Cells
Understanding the strategies for developing high yields of low immunogenic mRNA for downstream applications that require robust protein expression, such as in cell and gene therapy and vaccine applications, is critical. Explore this step-by-step guide to obtain the perfect mRNA for translation in cells—from 5’ capping and modified nucleosides to poly(A) tail length and dsRNA removal.
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Contact an mRNA Expert!
Need help designing your mRNA synthesis workflow to ensure lowest immunogenicity?
Contact a CELLSCRIPT™ mRNA Expert to discuss your needs!
