INCOGNITO™ iPSCMax™ Ψ-mRNA Reprogramming Mix

  • Reprogram fibroblasts with an optimized, proprietary mix of five pseudouridine (Ψ)-containing, dsRNA-depleted mRNAs.
  • Ultra-low immunogenicity Ψ-mRNA mix effectively avoids recognition by cellular innate immune response systems.
  • Reprogram fibroblasts with as few as five transfections and get colonies within two weeks.
  • Have peace of mind and save on downstream QC knowing no residual vector remains after reprogramming.

Product is for research use only (RUO)

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Catalog #
Size
Price
Quantity
Catalog #: IPY250906
Size: 6 wells
Price: $1,052.00
Catalog #: 2xIPY250906
Size: 12 wells
Price: $1,800.00

Product Description:

The INCOGNITO™ iPSCMax™ Ψ-mRNA Reprogramming Mix contains an optimized, proprietary mix of five pseudouridine (Ψ)-containing, double-stranded RNA (dsRNA)-depleted mRNAs encoding proteins with the ability to reprogram somatic cells, specifically fibroblasts, into induced pluripotent stem cells (iPSCs) with high efficiency. INCOGNITO™ mRNAs contain the uridine analog pseudouridine which is substituted for all uridines in the mRNA sequences. This substitution combined with dsRNA removal using the Min-Immune™ Gold dsRNA Removal system produces a mRNA mix with ultra-low immunogenicity that effectively avoids recognition by the receptors of the cellular innate immune response system.

The INCOGNITO™ iPSCMax™ Ψ-mRNA Reprogramming Mix is highly efficient, requiring as few as five transfections and producing colonies within two weeks. Due to the transient nature of mRNA in cells, INCOGNITO™ mRNAs leave no residual vector after reprogramming and pose no risk of genomic integration of the exogeneous factors, reducing downstream quality testing.

The Reprogramming Mix is available to reprogram either 6 wells (Cat. No. IPY250906) or 12 wells (Cat. No. 2xIPY250906) of a 6-well plate.

Cells reprogrammed into iPSCs can be differentiated into multiple cell types including neurons, cardiomyocytes and pancreatic islet cells and can be a source of cells for use in basic science studies, drug safety screening, development of immunotherapies and regenerative medicine therapies.

Product Performance:

Figure 1. INCOGNITO™ iPSCMax™ Reprogramming Timeline. Cells are reprogrammed into iPSCs with as few as five daily transfections, generating colonies
within two weeks.

 

 


Figure 2. Alkaline Phosphatase staining of iPSC colonies in a well of fibroblasts reprogrammed using the INCOGNITO™ iPSCMax™ Ψ-mRNA Reprogramming Mix.
The Reprogramming Mix results in high efficiency reprogramming producing colonies within two weeks.

 

 

Figure 3. Cells reprogrammed using the INCOGNITO™ iPSCMax™ Ψ-mRNA Reprogramming Mix stained positive for several pluripotency markers.

 

 

Figure 4. Cells reprogrammed using the INCOGNITO™ iPSCMax™ Ψ-mRNA Reprogramming Mix differentiated into the three germ layers and stained positive for markers of each respective germ layer.


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