RNase I
- Degrades single-stranded RNA cleaving after every base.
- Reactions result in individual nucleoside 3'-monophosphates.
Product is for research use only (RUO)
Product Description:
Ribonuclease I (RNase I) from E. coli degrades single-stranded RNA cleaving after every base resulting in individual nucleoside 3'-monophosphates. RNase I does not require divalent cations for activity and is not inhibited by ScriptGuard™ RNase Inhibitor, an RNase A-family inhibitor.
RNase I can be used for the following applications:
- Removal of RNA from DNA preparations.
- Mismatch detection in RNA:RNA and RNA:DNA hybrids.
Product Performance:
Figure 1. Ribosomal RNA is digested by RNase I. 30 μg of E. coli ribosomal RNA was treated with varying amounts of RNase I for 5 min at 37°C. Complete degradation was achieved using 2 U of RNase I (lane 2).
Figure 2. RNase I removes RNA from a DNA:RNA solution. 30 μg of a plasmid prep from an E. coli culture containing a 16.3-kb recombinant clone was treated with
1 U of RNase I for 15 min at 37°C. RNA is degraded, leaving the plasmid DNA intact (lane 2).
