RNase R
- Ideal for isolating circular RNA (circRNA) from total RNA preparations.
- Rapid workflow providing digestion of 5 µg of linear RNA with 1 µg of RNase R in just 30 minutes.
- Preserves circRNA, lariat intron RNA and dsRNA while digesting linear RNA and Y-structure RNA.
Product is for research use only (RUO)
Product Description:
Ribonuclease R (RNase R) from E. coli is a 3’→5’ exoribonuclease that digests linear RNA and Y-structure RNA but does not digest circular RNA (circRNA), lariat intron RNA, blunt-ended double-stranded RNA (dsRNA), dsRNA with 3’-overhangs <7 nt in length or single-stranded DNA. Highly structured linear cellular RNAs such as prokaryotic 23S and 16S ribosomal RNAs (rRNAs), small nuclear RNAs (snRNAs), transfer RNAs (tRNAs) and histone mRNAs are also not efficiently degraded by RNase R. 1 µg of RNase R will digest 5 µg of linear RNA in just 30 minutes at 37°C.
RNase R is ideal for circRNA isolation from total cellular RNA preparations for use in intron and splicing studies as well as for natural cellular circRNA identification. Additionally, RNase R is a crucial component in in vitro circRNA preparation cleanup and enrichment with both RNA Ligase-based or self-splicing permutated intron–exon sequence (PIE method)-based methodologies.
Product Performance:
Figure 1. A PIE-method RNA circularization prep was assessed on a denaturing gel before (UT) and after (T) RNase R treatment. The untreated sample lane shows different bands corresponding to linear RNA subproducts from the PIE-method. Following RNase R treatment, the linear RNA are digested, leaving only the circular RNA (red arrow) intact.
Figure 2. 5 µg of linear RNA was circularized with RNA Ligase to produce circular RNA (lane 2). The RNA ligation reaction product was treated with 1 µg of RNase R at 37°C for 30 minutes to enrich for circular RNAs (lane 3).
