Products for Translation in Cells

mRNA Production

T7 mScript™ Standard mRNA Production System (Manual | Safety Data Sheet)

Simply the best way to make in vitro mRNA. Combines the benefits of high-yield transcription with those of post-transcriptional capping and tailing all in one kit. See T7-FlashScribe Transcription Kit, ScriptCap m⁷G Capping System, ScriptCap 2'-O-Methyltransferase Kit and A-Plus Poly(A) Polymerase Tailing Kit on this page.

C-MSC11610	10 reactions	$330
C-MSC100625	25 reactions	$724

In Vitro Transcription

T7-FlashScribe™ Transcription Kit (Manual | Safety Data Sheet)

For the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 180 µg RNA from 1 µg DNA in
30 minutes.

C-ASF3507

50 reactions

$286

T7-Scribe™ Standard RNA IVT Kit (Manual | Safety Data Sheet)

For the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 150 µg RNA from 1 µg DNA in 2 hours.

C-AS3107

50 reactions

$267

SP6-Scribe™ Standard RNA IVT Kit (Manual | Safety Data Sheet)

For the production of in vitro transcribed RNA with SP6 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 90 µg RNA from 1 µg DNA in 2 hours.

C-AS3106

50 reactions

$278

Post-Transcriptional Capping

ScriptCap™ m⁷G Capping System (Manual | Safety Data Sheet)

For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, GTP and SAM. Cap 0 (N7-methyl-G) caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods. Use in conjunction with ScriptCap 2'-O-Methyltransferase to produce
Cap 1 caps, that which is found on most eukaryotic mRNAs.

C-SCCE0625

25 reactions

$450

ScriptCap™ 2'-O-Methyltransferase Kit (Manual | Safety Data Sheet)

For conversion of RNA containing Cap 0 caps to those containing Cap 1 caps, which are found on most eukaryotic mRNAs. Can be used simultaneously with ScriptCap m⁷G Capping System to produce virtually 100% Cap 1-capped RNA in a single reaction.

C-SCMT0625

25 reactions

$226

ScriptCap™ Cap 1 Capping System (Manual | Safety Data Sheet)

For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, ScriptCap 2'-O-Methyltransferase, GTP and SAM. Either Cap 0 or Cap 1 caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods.

C-SCCS1710	10 reactions	$220
C-SCCS2250	50 reactions	$1100

Co-Transcriptional Capping

MessageMAX™ T7 ARCA-Capped Message Transcription Kit V2 (Manual | Safety Data Sheet)

For co-transcriptional capping of IVT RNA with T7 RNA polymerase and Anti-Reverse cap analog (ARCA). A reaction produces 60 µg RNA from 1 µg DNA in 30 minutes. Reaction products are 80% correctly capped and 20% uncapped.

C-MMA60710

10 reactions

$340

For Kit V1 (Manual | Safety Data Sheet)

Post-Transcriptional Tailing

A-Plus™ Poly(A) Polymerase Tailing Kit (Manual | Safety Data Sheet)

For post-transcriptional poly(A) tail addition to RNAs. Allows tailing of mRNAs
in vitro, to lengths equivalent to or greater than those found in nature. This cannot be accomplished with co-transcriptional tailing methods.

C-PAP5104H

50 reactions

$226

Modified NTP In Vitro Transcription

INCOGNITO™ T7 Ψ-RNA Transcription Kit (Manual | Safety Data Sheet)

For the production of pseudouridine-containing, in vitro transcribed RNA with T7 RNA polymerase. Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 100 µg RNA from 1 µg DNA in 2 hours.

C-ICTY110510

10 reactions

$427

INCOGNITO™ SP6 Ψ-RNA Transcription Kit (Manual | Safety Data Sheet)

For the production of pseudouridine-containing, in vitro transcribed RNA with SP6 RNA polymerase. Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 60 µg RNA from 1 µg DNA in 2 hours.

C-ICSY110510

10 reactions

$423

INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit (Manual | Safety Data Sheet)

For the production of 5-methylcytosine- pseudouridine-containing, in vitro transcribed RNA with T7 RNA polymerase. 5mC- Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 100 µg RNA from 1 µg DNA in 2 hours.

C-ICTMY110510

10 reactions

$719

INCOGNITO™ T7 ARCA 5mC- & Ψ-RNA Transcription Kit (Manual | Safety Data Sheet)

For co-transcriptional capping of 5-methylcytosine- pseudouridine-containing,
in vitro transcribed RNA with T7 RNA polymerase and Anti-Reverse cap analog (ARCA). 5mC- Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 40 µg RNA from 1 µg DNA in 3 hours. Reaction products are 80% correctly capped and 20% uncapped.

C-ICTAMY110510

10 reactions

$876

mRNA Decapping

Cap-Clip™ Acid Pyrophosphatase (Manual | Safety Data Sheet)

For the removal of cap structures (decapping) from various RNAs (i.e. mRNAs) resulting in 5'-monophosphate RNAs. Can be substituted unit for unit, into any preexisting protocol for Tobacco Acid Pyrophosphatase (TAP) utilizing the same buffer.

C-CC15011H

100 units

$567

RNase Inhibition

ScriptGuard™ RNase Inhibitor (Manual | Safety Data Sheet)

Inhibits eukaryotic RNases A, B and C. Use as a safeguard against RNA degradation in any RNA-containing reaction.

C-SRI6310K

10,000 units

$278

The Products listed above are currently offered by CELLSCRIPT™ for research use only under the defined Terms and Conditions. To place an order, visit How To Order.

Contact CELLSCRIPT™ to inquire about customizations of existing products or manufacturing of products for other uses, such as therapeutics, which may require meeting regulatory standards, such as use of components that are not derived from animal origin.

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