RNase T1
- Specifically hydrolyzes the phosphodiester bond after guanosine bases
in RNA. - Cleavage activity is base-specific, enabling RNA mapping and
structure studies.
Product is for research use only (RUO)
Product Description:
Ribonuclease T1 (RNase T1) from Aspergillus oryzae specifically hydrolyzes the phosphodiester bond after guanosine bases in RNA. RNase T1 is not inhibited by ScriptGuard™ RNase Inhibitor, an RNase A-family inhibitor.
Due to its base-specific cleavage activity, RNase T1 can be used for the following applications:
- RNA mapping.
- RNA structure studies.
- RNA sequencing.
- Removal of RNA from DNA preparations.
Product Performance:
Figure 1. RNase T1 treatment results in base-specific cleavage. 100 picomoles of 25-nt ssRNA oligo containing a single G nucleotide at position 8 was treated with varying amounts of RNase T1 for 10 min at 37°C. RNase T1 cleavage is base-specific, with a 17-nt RNA band visible following treatment (lanes 2 and 3).
Figure 2. RNase T1 fully degrades ribosomal RNA. 12 μg of E. coli ribosomal RNA was treated with varying amounts of RNase T1 for 10 min at 37°C. Treatment with
2 U (lane 2) and 1 U (lane 3) of RNase T1 resulted in complete degradation of the RNA.
