A: The T7 mScript™ Standard mRNA Production System V2 produces capped mRNA with nearly 100% efficiency and with all the caps in the correct orientation. Direct incorporation of cap analog dinucleotides during traditional G-start co-transcriptional systems is considerably less efficient, where capping efficiencies cannot exceed 80%.
A: Unlike traditional G-start cap analog-based co-transcriptional reactions the T7 mScript™ Standard mRNA Production System V2 uses an enzymatic process to build cap structures onto the RNA transcripts which bypasses several of the limitations of the traditional G-start cap analog-based chemistry. These limitations include lower capping efficiencies, reduced in vitro transcription (IVT) reaction yields and limited IVT RNA lengths. The T7 mScript™ Standard mRNA Production System V2 includes ScriptCap™ Capping Enzyme, which contains all three enzymatic activities (mRNA triphosphatase, guanylyltransferase and guanine-7-methyltransferase) necessary to build a 5′-Cap 0 structure in vitro onto the RNA, ensuring nearly 100% capping with 100% proper cap orientation. The T7 mScript™ Standard mRNA Production System V2 also contains ScriptCap™ 2′-O-Methyltransferase, allowing the natural, translation boosting and self RNA identifying, Cap 1 structure to be built onto the RNA.
