Practical insights to improve IVT performance, RNA quality, and translation efficiency
Across mRNA workflows—from transcription through quality control—small differences in process can significantly impact
yield, consistency, and downstream performance.
Below are selected technical resources to help address common challenges in mRNA synthesis and analysis.
IVT Optimization
Improve yield, consistency, and transcription performance.
CELLSCRIPT™’s dual workflow combining N1-methyl-pseudouridine (N1meΨ) modification with enzymatic dsRNA removal achieves high mRNA performance.
Explore essential steps and design strategies to optimize mRNA synthesis for therapeutic success.
Róisin McGuigan, Editor, Nucleic Acid Insights, speaks to Ryan Lahr, Senior R&D Scientist, CELLSCRIPT™, about design, production, and quality control of synthesized mRNA for translation in cells.
Capping & Translation Efficiency
Improve expression and downstream performance.
Optimizing mRNA synthesis, modification, and purification enhances translational efficiency.
Pairing chemical uridine modification with enzymatic dsRNA removal yields high-quality, immunogenicity-free mRNA.
RNA Quality & dsRNA Reduction
Improve purity and reproducibility
Featured at RNATx Symposium. Messenger RNA (mRNA) and self-amplifying RNA (saRNA) technologies continue to show transformative potential across therapeutic fields, including engineered cell therapies and cell reprogramming, where precise control over immunogenicity is critical for success.
Featured at ASGCT 2026. Messenger RNA (mRNA) technologies continue to show transformative potential across therapeutic fields, including engineered cell therapies and cell reprogramming, where precise control over immunogenicity is critical for success.
We discuss the impacts of dsRNA in RNA production and strategies to enhance translation and reduce immunogenicity.
Featured at TIDES USA 2026. EZ-QC™ mRNA Assay Kits produce results comparable to LC-MS and offer a convenient, flexible, and cost-effective approach to mRNA QC.
Complete mRNA Synthesis
Create post-transcriptionally
5′-capped and 3′-poly(A) tailed mRNA with greatly reduced dsRNA content to lower innate immunogenicity.
In Vitro Transcription
mRNA transcription using canonical or modified nucleotides.
5' Capping
Post-transcriptional addition of Cap 0 or Cap 1 with ~100% efficiency.
3' Poly(A) Tailing
Post-transcriptional tailing of mRNAs to lengths of 20-300+ A’s.
dsRNA Removal
Easy and scalable enzymatic double-stranded RNA removal from IVT RNA samples.
mRNA Quality Control
Ideal for in-process or final product testing to measure mRNA
5’-capping efficiency or 3’-poly(A) tail length from
20-300+ A’s.
Additional Resources
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